Mastering Your Lamotte Dissolved Oxygen/Temperature TRACER: A Comprehensive Guide

25, May 2024

Unlock the full potential of your Lamotte Dissolved Oxygen/Temperature TRACER with this detailed operational manual. From initial setup to advanced calibration techniques, we cover everything you need to know to ensure accurate measurements and reliable performance.


The Lamotte Dissolved Oxygen/Temperature TRACER is a sophisticated instrument designed to provide precise measurements of dissolved oxygen levels and temperature in aquatic environments. Whether you’re a seasoned scientist or a novice enthusiast, mastering the operation of this device is essential for obtaining accurate data and maximizing its capabilities.


  1. Filling the Electrode: Before the initial use, the electrode must be filled with the supplied electrolyte solution to ensure optimal performance.
  2. Powering the Tracer: Utilizing four CR2032 Lithium Ion Batteries, the Tracer can be easily powered on or off with the press of a button. The auto power-off feature conserves battery life, shutting the Tracer off after 10 minutes of inactivity.
  3. Startup Polarization Period: Upon initial power-up, the electrode requires a polarization period of approximately three minutes for accurate measurements. Subsequent power-ups within a seven-day period maintain electrode polarization, enabling immediate measurements.
  4. Turn-On Diagnostics: The Tracer conducts self-diagnostics upon power-up, ensuring accurate readings and optimal performance.
  5. Changing Settings: Users can adjust temperature units, salinity compensation, altitude compensation, and measurement units to suit their specific requirements.
  6. Auto-Power Off Feature: The Tracer includes an auto-power off feature to conserve battery life. This feature can be disabled for extended usage.
  7. Low Battery Indicator: A low battery indicator alerts users when battery voltage falls below the operating threshold, ensuring uninterrupted operation.
  8. Storing and Recalling Readings: The Tracer can store up to 25 readings, allowing users to easily recall and analyze data as needed.
  9. Clearing Stored Readings: Users can clear stored readings to free up memory for new data collection.


  1. Membrane Cap Assembly: Proper assembly and filling of the membrane cap are essential for accurate measurements. Careful attention to detail during this process ensures optimal performance.
  2. Polarization: If the Tracer has not been used for seven days or longer, electrode polarization may be required before accurate measurements can be obtained.
  3. Calibration: Daily calibration is recommended to maintain accuracy. Optional zero oxygen calibration can further enhance measurement precision.

Dissolved Oxygen Measurement

  1. Sample Preparation: Proper electrode placement and sample stirring are crucial for obtaining accurate dissolved oxygen measurements.
  2. Stabilization Time: Allow sufficient time for the temperature of the probe to stabilize before taking a reading to ensure accuracy.
  3. Constant Movement: The polarographic sensor requires constant movement of the sample across the membrane for accurate measurements.


With its advanced features and precise measurements, the Lamotte Dissolved Oxygen/Temperature TRACER is a valuable tool for researchers, scientists, and enthusiasts alike. By following the guidelines outlined in this manual, you can master the operation of your Tracer and unlock its full potential for aquatic research and analysis.