Accurate determination of ethanol content is crucial across various industries, including beverage production and environmental monitoring. Traditional methods, such as gas chromatography and distillation, often require expensive equipment, extensive sample preparation, and skilled personnel. The NANOCOLOR® Ethanol Tube Test offers a streamlined, cost-effective alternative for precise ethanol measurement.
The Science Behind the Test
The NANOCOLOR® Ethanol Tube Test utilizes an enzymatic reaction for the photometric determination of ethanol. In this process, the enzyme alcohol oxidase catalyzes the oxidation of ethanol to acetaldehyde and hydrogen peroxide. The generated hydrogen peroxide then reacts with a chromogen in the presence of peroxidase, resulting in the formation of a blue dye. The intensity of this blue coloration is directly proportional to the ethanol concentration and is measured photometrically.
Key Features and Benefits
Versatility: Designed for analyzing ethanol content in various samples, including spirits, beer, and fruit juices.
Precision: The test offers a measuring range from 0.10 to 1.00 g/L of ethanol (0.013 to 0.130 Vol. % EtOH), ensuring accurate results for both low and high ethanol concentrations.
Compatibility: Optimized for use with NANOCOLOR® photometers, including the 500 D, PF-12 Plus, and all NANOCOLOR® spectrophotometers, facilitating seamless integration into existing laboratory workflows.
Efficiency: The pre-dosed reagents in 16 mm tubes allow for direct measurement, reducing preparation time and minimizing exposure to chemicals.
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Simplified Procedure
Sample Preparation: Filter the sample to remove any particulate matter.
Reagent Addition: Add the sample to the pre-dosed NANOCOLOR® Ethanol test tube.
Incubation: Allow the enzymatic reaction to proceed for a specified time.
Measurement: Insert the test tube into the compatible photometer to measure the absorbance, which correlates to the ethanol concentration.
This straightforward protocol enables rapid analysis, making it ideal for routine quality control and on-site testing.
Considerations
While the NANOCOLOR® Ethanol Tube Test is robust, it’s essential to be aware of potential interferences. Strong oxidizing agents present in the sample can lead to elevated readings. Therefore, it’s advisable to assess the sample matrix for such substances prior to analysis.
The NANOCOLOR® Ethanol Tube Test revolutionizes ethanol measurement by combining accuracy, efficiency, and user-friendliness. Its enzymatic approach ensures specificity, while the streamlined procedure reduces operational complexities. For industries and laboratories seeking reliable ethanol analysis without the need for sophisticated instrumentation, this test offers an exemplary solution.